Step-by-Step Reconstitution Protocol
Gather your equipment
You will need: one vial of MOTS-C 10mg (lyophilised), bacteriostatic water (BAC water), a sterile syringe and needle, an alcohol swab, and a clean work surface. Ensure all equipment is sterile.
Calculate your target concentration
The standard reconstitution for MOTS-C 10mg is to add 2.0 mL of bacteriostatic water, yielding a concentration of 5000 mcg/mL. Use our peptide calculator to adjust for different doses.
Clean the vial stopper
Wipe the rubber stopper of the MOTS-C vial with an alcohol swab. Allow to air-dry for 10–15 seconds before inserting the needle.
Draw and inject the bacteriostatic water
Draw 2.0 mL of bacteriostatic water into the syringe. Insert the needle into the MOTS-C vial and inject the BAC water slowly against the inner glass wall — do not inject directly onto the lyophilised powder. This prevents foaming and peptide degradation.
Dissolve the peptide
Gently swirl the vial until the lyophilised powder is fully dissolved. Do not shake or vortex. MOTS-C should dissolve within 30–60 seconds. The solution should be clear — if it appears cloudy, check your BAC water is sterile and at room temperature.
Label and store
Label the vial with the reconstitution date and concentration. Store lyophilised at −80 °C for long-term stability. Short-term storage at −20 °C acceptable. Reconstituted: 4 °C, use within 14 days. Use within the recommended timeframe to ensure maximum peptide integrity.
Concentration Reference Table
| BAC Water Added | Concentration (mcg/mL) | mg/mL |
|---|---|---|
| 1 mL | 10000 mcg/mL | 10.0 mg/mL |
| 2.0 mL (recommended) | 5000 mcg/mL | 5.0 mg/mL |
| 4.0 mL | 2500 mcg/mL | 2.50 mg/mL |
Use our interactive peptide reconstitution calculator for custom dose and syringe unit conversions.
Storage After Reconstitution
Store lyophilised at −80 °C for long-term stability. Short-term storage at −20 °C acceptable. Reconstituted: 4 °C, use within 14 days.