Step-by-Step Reconstitution Protocol
Gather your equipment
You will need: one vial of NAD+ 1000mg (lyophilised), bacteriostatic water (BAC water), a sterile syringe and needle, an alcohol swab, and a clean work surface. Ensure all equipment is sterile.
Calculate your target concentration
The standard reconstitution for NAD+ 1000mg is to add 10.0 mL of bacteriostatic water, yielding a concentration of 100000 mcg/mL. Use our peptide calculator to adjust for different doses.
Clean the vial stopper
Wipe the rubber stopper of the NAD+ vial with an alcohol swab. Allow to air-dry for 10–15 seconds before inserting the needle.
Draw and inject the bacteriostatic water
Draw 10.0 mL of bacteriostatic water into the syringe. Insert the needle into the NAD+ vial and inject the BAC water slowly against the inner glass wall — do not inject directly onto the lyophilised powder. This prevents foaming and peptide degradation.
Dissolve the peptide
Gently swirl the vial until the lyophilised powder is fully dissolved. Do not shake or vortex. NAD+ should dissolve within 30–60 seconds. The solution should be clear — if it appears cloudy, check your BAC water is sterile and at room temperature.
Label and store
Label the vial with the reconstitution date and concentration. Store lyophilised at −20 °C, protect from moisture. Reconstituted: 4 °C, protect from light, use within 7 days. Use within the recommended timeframe to ensure maximum peptide integrity.
Concentration Reference Table
| BAC Water Added | Concentration (mcg/mL) | mg/mL |
|---|---|---|
| 1 mL | 1000000 mcg/mL | 1000.0 mg/mL |
| 10.0 mL (recommended) | 100000 mcg/mL | 100.0 mg/mL |
| 20.0 mL | 50000 mcg/mL | 50.00 mg/mL |
Use our interactive peptide reconstitution calculator for custom dose and syringe unit conversions.
Storage After Reconstitution
Store lyophilised at −20 °C, protect from moisture. Reconstituted: 4 °C, protect from light, use within 7 days.